The western blot provides information on whether or not the ERB2 protein is bound to another protein. cation-anion exchange, size exclusion, nickel, etc.)ģ. Review protein purification techniques and different types of purification columns (i.e. The other tags are generally not used in experiments, and they would not be used for a nickel column as His-tags are standard. His6 tags are added to proteins (at either the N- or C-terminus) to allow the tagged proteins to bind to columns (choice A is correct). Review hydrophobic effect and thermodynamics. Hydrogen bonding contributes weakly, if at all (choice B is incorrect). Disulfide and salt bridges are formed within a protein and not between two different proteins (choices A and D are incorrect). The hydrophobic effect provides the most energy stabilization for interactions between proteins (choice C is correct). Which of the following conclusions is best supported by Figure 2?Ī) An RNA molecule plays a role in the AGO2-ERB2 interactionī) ERB2 is not expressed when RNase is presentĬ) AGO2 binds ERB2 more tightly in the absence of RNAĭ) AGO2 is upregulated when RNase is present Answer key for practice passage #1ġ. Why do researchers include the input lanes in both RNase conditions in Figure 2?Ī) To determine the concentration of AGO2 before the experimentī) To ensure that equal amounts of AGO2 were present before the experimental treatmentĬ) To measure the amount of ERB2 complex without RNaseĭ) To increase the likelihood of binding events for later conditionsĥ.
To interpret the results of Figure 1, the researchers must assume all of the following EXCEPT:Ī) Equal concentrations of RNase were used in the RNase + lanes.ī) Equal concentrations of nuclear extract were loaded in all of the lanes.Ĭ) Equal amounts of ERB in complex with other proteins form in all of the lanes after RNase treatment.ĭ) The antibody for ERB is specific to the protein at a site distinct from protein interaction sites.Ĥ. Researchers decide to purify ERB2 using a nickel column. Which of the following most likely contributes the greatest amount stability to the ERB2-AGO2 interaction?Ģ. You’ll notice that each explanation has suggestions for what you should review if you miss a question. Use the following three passages and five standalone questions to test your ability to apply your biochemistry knowledge to real, MCAT-style passages. From enzymes to experimental techniques (link experimental techniques page), biochemistry questions will make up a portion of both your chemistry/physics and biology/biochemistry section scores.
But, you find that content review is not enough to score well on the MCAT! You need to be able to apply your content knowledge to the rest of the exam.īiochemistry is one of the most heavily tested subjects on the MCAT. You’ve created a perfect study schedule and have started diving into your content review. Your test date looms in the future, and you’re in the thick of your test preparation. Achieving a great MCAT score can greatly increase your chances of hearing “We’re pleased to inform you…” from the medical school of your dreams. You are ready to sit down and study for the MCAT, which is one of the most important pieces of your medical school application.
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